: We aimed to evaluate the effect of insulin resistance on serum effect of IR on serum OPG and trace elements levels in diabetic nephropathy patients in province of Basrah-Iraq. From 63 volunteers who suffering from T2DM, 31 patients with nephropathy and 32 patients without nephropathy, while 33 normal voleteers were taken as controls. Their fasting insulin hormone and osteoprotegerin were determined by ELISA methods. BMI, glucose urea, creatinine, GFR and Cr.Cl., and homeostasis model assessment for determined of insulin resistance (IR). Mg and Zn were measured by flame atomic absorption spectrometry, while Se in whole blood was determined using hydride generation method. A non-significant change (p>0.05) was seen in the level of BMI, significantly decreased (p<0.01) in levels of Se, Zn and Mg, while a higher significantly changes (p<0.01) were seen in the levels of glucose, insulin, HOMA-IR, urea, creatinine in subjects of type 2 diabetic patients with and without nephropathy, as compared to healthy group. On the other hand, levels of OPG, GFR and Cr.Cl., were highly significantly (p<0.01) changes in diabetic patients with nephropathy and significantly (p<0.05) changes in patients without nephropathy, compared to the healthy control. Elevated serum OPG and decreased trace elements (Se, Zn and Mg) levels are strongly associated with BMI, insulin resistance and physical activity which can be used as a biomarker of renal dysfunction in diabetic nephropathy thus decreasing the mortality and morbidity.