: Based on the comprehensive stem cells (SCs) research results, many researchers have assumed that the cells could possibly create treatment and management for several diseases including cardiovascular disease, cancers, and igniting optimism of developing SCs replacement treatment in a health setting. Nurses can play a significant role in giving information to the community about SCs. Therefore, the knowledge given from nurses to patients must be accurate and balanced. Evaluate maternity nurses’ knowledge and attitude regarding SCs and cord blood collection. Methods: A cross-sectional study was carried on a convenience sample of maternity nurses (n= 198) worked in maternity and children hospital (MCH), Najran city. A self-administrated questionnaire was used which contains three main parts; part I: Demographic data, part II: knowledge assessment toward SCs and cord blood collection, and part III: Modified Likert scale to assess nurses’ attitude as regard to SCs and cord blood collection. About two-third (69.7%) of nurses had fair knowledge about SCs and cord blood collection and only 30.3% of them had good knowledge. About 43.9% of them exhibited positive attitude regarding SCs. Moreover, there was a significant relationship (FET= 9.274, p= 0.009) between maternity nurses' knowledge and their attitude. Maternity nurses in MCH Najran had fair knowledge and a positive attitude toward SCs and cord blood collection. Consequently, the study recommends implementing in-services training programs related to SCs and cord blood collection for the nurses to enhance their information and motivate a more positive attitude.