: A novel coronavirus infection (known as “COVID-19”) is an emerging infectious disease that has been declared as public health emergency of international concern in March 2020. The severity of COVID- 19 varies, from mild to severe. Lack of information, unfavorable attitude, going into crowded places, and inappropriate mask utilization could lead to exacerbated transmission of the pandemic. To assess the level of knowledge, attitudes, and practices (KAP) toward COVID-19 prevention among university students during the COVID-19 outbreak. A cross-sectional study was conducted between November 1st and December 1st, 2020, at the Arab American University of Palestine. An online questionnaire was sent to all registered undergraduate students via the student's websites in each faculty and Facebook groups. The questionnaire was composed of two parts: Sociodemographic part and knowledge, attitude, and practice (KAP) towards Covid-19 part. Descriptive and differential statistics were used as statistical analysis. The results of the 12 Knowledge questions responses showed that percentage of the correct answers were between 45.3% and 95%. Knowledge scores significantly differed across genders, residency type, and among different colleges. Attitudes of students have been assessed, 97.2% expressed believe and confidence in controlling COVID -19 infection through handwashing. Regarding practice, respondents have complied with some preventative measures (77%). This study showed a higher knowledge, higher positive attitudes toward COVID-19. Practices of university students toward the virus were extremely cautious, but not optimal, and poor practice for certain recommended preventive measures was noticed..