Abstract : The impact of extraction condition of antioxidant polyphenol and chlorophyll from species Hydrocotyle asiatica grown in Vietnam was presented in the current study. The extraction condition was studied composed of solvent concentrations (60 to 100 with jump step (δ) of 5) and solvent pH (2 to 10 with δ of 1). Besides the content of polyphenol and chlorophyll, the target function of antioxidant activity was also evaluated based on total antioxidant activity and reducing power activity. The results showed that polyphenol and chlorophyll content and antioxidant activity from Hydrocotyle asiatica were affected by the solvent concentration and solvent pH significantly (p<0.05). The content of polyphenol (3.958±0.028 mg gallic acid equivalent/g DW) and chlorophyll (2.412±0.057 mg/g DW), and the antioxidant activity composed of total antioxidant activity (2.077±0.022 mg ascorbic acid/g DW) and reducing power activity (11.366±0.53 mg FeSO4 equivalent/g DW), was the highest at 95% ethanol and solvent (pH 5), compared to other conditions. Polyphenol, chlorophyll content, and antioxidant activity were changed according to the model of level 2 under the impact of solvent concentration and solvent pH. The results were valuable in the antioxidant polyphenol and chlorophyll extraction for applicating functional food and pharmaceuticals..
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Abstract : Surgical stress is an immediate aftermath of most surgical procedures which may directly/indirectly prolong recuperation and healing process of the animal. More stress response also emanates from the type of suture material and pattern used for the incision closure. Researchers are looking for materials that can lessen this stress. Serum cortisol measurement is one of the reliable markers of stress response. With the introduction of medical grade cyanoacrylate into clinical practice as a skin closure material, a number of promising outcomes have been reported by some researchers and its potential alternative to conventional sutures. This study evaluates surgical stress response in skin incisions closed with N-butyl Cyanoacrylate and Nylon while monitoring serum cortisol levels in Sahel bucks. Ten clinically healthy bucks (male goats) with mean age of 9.7±1.33 months with mean weight 13.2±1.31kg were randomly separated into two groups (A and B) of five bucks each. The bucks were prepared for surgery and skin incisions made were closed using N-butyl Cyanoacrylate and Nylon for group A and B respectively. Blood samples were taken before and after the procedure at 0, 5, 8, 24, 48 and 72 hours where serum cortisol levels were quantitatively evaluated. Serum cortisol levels were seen to rise in incisions closed with Nylon compared to those closed with N-butyl Cyanoacrylate..
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Abstract : Mass in the right iliac fossa has a very high incidence rate among the cases of lump in abdomen. It’s a diagnostic and therapeutic challenge to surgeons due to the detailed anatomy of the region and varied etiology. To study the demographic profile of right iliac fossa mass. In this prospective comparative study, total 50 cases were studied for duration of 2 years. Detailed demographic data was obtained after confirming the diagnosis. Necessary management was done. Comparison of all the data with various demographic parameters was done to reach conclusion. Maximum cases were from age group of 31-40 yrs with mean age was 40.76 years and male predominance (58%). Appendicular lump (28%) was most common diagnosis among study subjects followed by ileocaecal tuberculosis (14%). Farmers and labourers were more prone to develop mass in right iliac fossa. Mass in right iliac fossa is commonly seen in males of age group 31 to 40 years with low socioeconomic status. Appendicular mass is most common pathology in RIF region although ileocaecal tuberculosis also have good incidence rate. Due to its varied presentation, high index of suspicion is absolutely necessary for diagnosis and management of right iliac fossa mass..
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Abstract : Oral cancer is the most common type of cancer affecting large population in India. Tobacco consumption including smoking and smokeless tobacco are some of the risk aspects for the incidence of oral cancer. Detection of premalignant oral lesions reduces the overall mortality from the disease and from the treatment procedures of oral cancer. Toluidine Blue is an acidophilic dye which has affinity for DNA and RNA present excess in dysplastic cells. In our study we have used Toluidine Blue as diagnostic test to detect premalignant oral lesions. Prospective study done in Dr. D. Y. Patil Medical College, Hospital &Research Centre, Pimpri, Pune, Under Department of General Surgery from July 2019 to August 2021. Patients presenting with oral lesions and who has risk factors for developing oral cancer were stained with an aqueous solution of 1% Toluidine blue, stained lesions were subjected to biopsy and histology. Sensitivity, Specificity and diagnostic accuracy of Toluidine Blue in detecting oral premalignant lesions among our study sample was 96.15%, 77.78%, 91% respectively. Toluidine Blue stain aids in diagnosing Premalignant Oral Lesions with good sensitivity and specificity. It is also simple, low cost and easily available..
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Abstract : Antibiotic resistance of an organism associated dental infections in patients with Orofacial cleft is more common over the past two decades due to irrational usage of antibiotics. It seems to be a major challenge to dental professionals for treating patients. Hence, short term antibiogram study was performed by using methanolic extract of Garcinia imberti bourd against multidrug-resistant (MDR), periodontal pathogens as well as root canal isolates such as Staphylococcus sps, Enterococcus sps and some gram negative isolates porphyromonas sps in dental sample. Samples, from which yielded bacterial strains were subjected into antibiotic susceptibility profile to detect the Multi Drug Resistance, Xtreme Drug Resistance, and Pan Drug Resistance bacteria. Out of 50 samples tested,227 isolates obtained and from this 100 (35.68) MDR 50 (17.18)XDR and PDR 3(1.3% ). The most common MDR root canal isolate was Enterococcus faecalis (66%), followed by rare Staphylococcus sciuri species 46% (13.33%) resulted from periodontics infected cleft palate patient. PDR resistance only observed in gram positive isolates of cleft palate cases. Antibacterial activity of methanolic fraction extract of novel medicinal plant Garcinia imberti Bourd had shown to exhibit potent activity against the drug resistant isolates Enterococcus faecalis (P<0.001) and Staphylococcus sciuri (P<0.001). Enterococcus faecalis showing Resistant to vancomycin (72.56%) but moderate resistance showed against gentamicin (35.6%) followed by Staphylococcus sciuri exhibited the resistant (64.43%) and (25.16%) respectivelyA significantly high resistance was noted to the beta-lactam group of antimicrobials, fluoroquinolones and cotrimoxazole, both by the gram-negative bacilli (GNB) as well as gram-positive cocci (GPC. Methanolic extract fraction of Garcinia imberti bourd could be used for the discovery of phytochemicals with drastic efficacy to battle against multidrug resistant oral isolates..
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